The immense progress in the field of therapy options over the past several years has significantly improved the chances of cure for patients with colon cancer. However, these new approaches, such as immunotherapies, require precise diagnosis so that they can be specifically tailored to the individual. Researchers at the Centre for Protein Diagnostics PRODI at Ruhr University Bochum, Germany, are using artificial intelligence in combination with infrared imaging to optimally tailor colon cancer therapy to individual patients. The label-free and automatable method can complement existing pathological analyses. The team led by Professor Klaus Gerwert reports in the “European Journal of Cancer” in January 2023.
Deep insights into human tissue within one hour
The PRODI team has been developing a new digital imaging method over the past few years: the so-called label-free infrared (IR) imaging measures the genomic and proteomic composition of the examined tissue, i.e. it provides molecular information based on the infrared spectra. This information is decoded with the help of artificial intelligence and displayed as false-colour images. To do this, the researchers use image analysis methods from the field of deep learning AI.
In cooperation with clinical partners, the PRODI team was able to show that the use of deep neural networks makes it possible to reliably determine the so-called microsatellite status, a prognostically and therapeutically relevant parameter, in colon cancer. In this process, the tissue sample goes through a standardised, user-independent, automated process and enables a spatially resolved differential classification of the tumour within one hour.
Indication of the effectiveness of therapies
In classical diagnostics, microsatellite status is determined either by complex immunostaining of various proteins or by DNA analysis.
“15 to 20 per cent of colon cancer patients show microsatellite instability in the tumour tissue,” says Professor Andrea Tannapfel, head of the Institute of Pathology at Ruhr University. “This instability is a positive biomarker indicating that immunotherapy will be effective.”
With the ever-improving therapy options, the fast and uncomplicated determination of such biomarkers is also becoming more and more important. Based on IR microscopic data, neuronal networks were modified, optimised, and trained at PRODI to establish label-free diagnostics. Unlike immunostaining, this approach does not require dyes and is significantly faster than DNA analysis.
“We were able to show that the accuracy of IR imaging for determining microsatellite status comes close to the most common method used in the clinic, immunostaining,” says Stephanie Schörner, PhD student. “Through constant further development and optimisation of the method, we expect a further increase in accuracy,” adds Dr Frederik Großerüschkamp.
Cooperation partners
This project was made possible by a long-standing, intensive cooperation between the Institute of Pathology at Ruhr University (Prof. Tannapfel), the Clinic for Haematology and Oncology at the St. Josef Hospital, Clinical Centre of Ruhr University (Professor Anke Reinacher- Schick) and the Centre for Protein Diagnostics (Prof. Gerwert).
The PRODI researchers were able to access the ColoPredict Plus 2.0 molecular registry, a non-interventional, multi-centre registry study for patients with early-stage colorectal cancer, to develop this diagnostic approach.
“The ColoPredict registry also enables a more targeted therapy for patients through the targeted analysis of biomarkers. Thus, the registry recently serves as a study platform for precision oncology approaches,” says Prof. Reinacher-Schick.
In addition to providing tissue samples, the registry offers a sound database of prognostically and therapeutically relevant baseline characteristics.
“In such a project, it is of immense importance to be able to draw on an excellent cohort and pathological expertise,” emphasises Prof. Gerwert.
“Our work on the classification of microsatellite status in colon cancer patients is based on one of the largest cohorts we have published to date and clearly demonstrates the potential for use in translational cancer research,” says Prof. Tannapfel.
Reference:
Klaus Gerwert, Stephanie Schörner, Frederik Großerueschkamp, et. al. Fast and label-free automated detection of microsatellite status in early colon cancer using artificial intelligence integrated infrared imaging. European Journal of Cancer, 28 January 2023.
doi: https://doi.org/10.1016/j.ejca.2022.12.026
